Journal: Biomolecules & Therapeutics

Article Title: Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells

doi: 10.4062/biomolther.2015.023

Figure Lengend Snippet: Effect of imperatorin on cell viability (A), degranulation (B), intracellular Ca 2+ level (C), and PLCγ1 phosphorylation (D) in IgE/Ag-stimulated BMMC. Cell viability was measured using an MTS assay (A). BMMC sensitized overnight with IgE were pre-incubated with the indicated concentrations of imperatorin for 1 h and stimulated with DNP-HAS (Ag, 100 ng/ml) for 15 min. β-HEX release into the supernatant was determined (B) and BMMC were used for Western blotting (D). IgE-sensitized BMMC pretreated with FluoForte TM Dye-Loading Solution were seeded into 96-well microplates, pre-incubated with imperatorin, and stimulated with DNP-HAS. Relative Ca 2+ levels were measured using Multilabel Plate Reader (C). The values are expressed as the means ± S.E.M. of three different samples. * p <0.05, ** p <0.01, *** p <0.001, significantly different from the control as determined by one-way ANOVA.

Article Snippet: Imperatorin was purchased from ChromaDex (Irvine, CA, USA).

Techniques: Phospho-proteomics, MTS Assay, Incubation, Western Blot, Control

Journal: Biomolecules & Therapeutics

Article Title: Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells

doi: 10.4062/biomolther.2015.023

Figure Lengend Snippet: Effect of imperatorin on LTC 4 generation (A), 5-LO (B), and cPLA 2 phosphorylation (C). BMMC sensitized overnight with IgE were pre-incubated with the indicated concentrations of imperatorin for 1 h and stimulated with DNP-HAS (Ag, 100 mg/ml) for 15 min. LTC 4 release into the supernatant was determined using an enzyme immunoassay kit (A) and the cells were used for Western blot analysis to detect the cytosolic and nuclear fraction of 5-LO (B), and total cell lysates for cPLA 2 (C). The values are expressed as the means ± S.E.M. of three different samples. * p <0.05, ** p <0.01, *** p <0.001, significantly different from the control as determined by one-way ANOVA.

Article Snippet: Imperatorin was purchased from ChromaDex (Irvine, CA, USA).

Techniques: Phospho-proteomics, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: Biomolecules & Therapeutics

Article Title: Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells

doi: 10.4062/biomolther.2015.023

Figure Lengend Snippet: Possible inhibitory mechanisms of imperatorin on degranulation and eicosanoid generation in mast cells.

Article Snippet: Imperatorin was purchased from ChromaDex (Irvine, CA, USA).

Techniques:

Journal: Biomolecules & Therapeutics

Article Title: Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells

doi: 10.4062/biomolther.2015.023

Figure Lengend Snippet: Suppressive effect of imperatorin on COX-2-dependent PGD 2 production (A, B) and Akt/IKK/IκBα/NF-κB activation (C–F). BMMC sensitized overnight with IgE were pre-incubated with aspirin for 2 h and then stimulated with DNP-HAS (Ag, 100 mg/ml) for 7 h with the indicated concentrations of imperatorin. PGD 2 release into the supernatant was determined using an enzyme immunoassay kit (A) and the cells were used for Western blotting to detect COX-2 protein (B). BMMC were pre-incubated with the indicated concentrations of imperatorin for 1 h and stimulated with DNP-HAS (Ag, 100 mg/ml) for 30 min. Total cell lysates and nuclear protein fractions were prepared and used for Western blot analysis (C–F). The values are expressed as the means ± S.E.M. of three different samples. * p <0.05, ** p <0.01, *** p <0.001, significantly different from the control as determined by one-way ANOVA.

Article Snippet: Imperatorin was purchased from ChromaDex (Irvine, CA, USA).

Techniques: Activation Assay, Incubation, Enzyme-linked Immunosorbent Assay, Western Blot, Control

Journal: Biomolecules & Therapeutics

Article Title: Imperatorin Suppresses Degranulation and Eicosanoid Generation in Activated Bone Marrow-Derived Mast Cells

doi: 10.4062/biomolther.2015.023

Figure Lengend Snippet: Suppressive effect of imperatorin on activation of MAPKs. BMMC sensitized overnight with IgE were pre-incubated with the indicated concentrations of imperatorin for 1 h and stimulated with DNP-HAS (Ag, 100 mg/ml) for 15 min. The cells were harvested and cell lysates were prepared for measurement of total or phosphorylated ERK1/2 (A), JNK (B), and p38 (C) by Western blot analysis. Results are representative of three separate experiments. The values are expressed as the means ± S.E.M. of three different samples. ** p <0.01, *** p <0.001, significantly different from the control as determined by one-way ANOVA.

Article Snippet: Imperatorin was purchased from ChromaDex (Irvine, CA, USA).

Techniques: Activation Assay, Incubation, Western Blot, Control